Regulatory

Part:BBa_K137032:Design

Designed by: Allen Lin   Group: iGEM08_Caltech   (2008-07-21)

constitutive promoter with (C)12 between -10 and -35 elements


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

With 12 C repeats between the -10 and -35 elements, the distance between the two elements is optimal for the sigma factor to bind to the promoter. Thus, the promoter should be in the 'on' state.

Source

This part was PCR amplified from two synthesized primers that bound to each other.

References